Nicotinic Receptor Subunits Atlas in the Adult Human Lung.
Fiche publication
Date publication
octobre 2020
Journal
International journal of molecular sciences
Auteurs
Membres identifiés du Cancéropôle Est :
Pr POLETTE Myriam, Dr DORMOY Valerian
Tous les auteurs :
Diabasana Z, Perotin JM, Belgacemi R, Ancel J, Mulette P, Delepine G, Gosset P, Maskos U, Polette M, Deslée G, Dormoy V
Lien Pubmed
Résumé
Nicotinic acetylcholine receptors (nAChRs) are pentameric ligand-gated ion channels responsible for rapid neural and neuromuscular signal transmission. Although it is well documented that 16 subunits are encoded by the human genome, their presence in airway epithelial cells (AECs) remains poorly understood, and contribution to pathology is mainly discussed in the context of cancer. We analysed nAChR subunit expression in the human lungs of smokers and non-smokers using transcriptomic data for whole-lung tissues, isolated large AECs, and isolated small AECs. We identified differential expressions of nAChRs in terms of detection and repartition in the three modalities. Smoking-associated alterations were also unveiled. Then, we identified an nAChR transcriptomic print at the single-cell level. Finally, we reported the localizations of detectable nAChRs in bronchi and large bronchioles. Thus, we compiled the first complete atlas of pulmonary nAChR subunits to open new avenues to further unravel the involvement of these receptors in lung homeostasis and respiratory diseases.
Mots clés
airway epithelial cells, lung, nicotinic receptors
Référence
Int J Mol Sci. 2020 Oct 9;21(20):