Modeling of Cell-Mediated Self-Assembled Colloidal Scaffolds.
Fiche publication
Date publication
octobre 2020
Journal
ACS applied materials & interfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Dias CS, Custódio CA, Antunes GC, Telo da Gama MM, Mano JF, Araújo NAM
Lien Pubmed
Résumé
A critical step in tissue engineering is the design and synthesis of 3D biocompatible matrices (scaffolds) to support and guide the proliferation of cells and tissue growth. The most existing techniques rely on the processing of scaffolds under controlled conditions and then implanting them , with questions related to biocompatibility and implantation that are still challenging. As an alternative, it was proposed to assemble the scaffolds through the self-organization of colloidal particles mediated by cells. To overcome the difficulty to test experimentally all the relevant parameters, we propose the use of large-scale numerical simulation as a tool to reach useful predictive information and to interpret experimental results. Thus, in this study, we combine experiments, particle-based simulations, and mean-field calculations to show that, in general, the size of the self-assembled scaffold scales with the cell-to-particle ratio. However, we have found an optimal value of this ratio, for which the size of the scaffold is maximal when the cell-cell adhesion is suppressed. These results suggest that the size and structure of the self-assembled scaffolds may be designed by tuning the adhesion between cells in the colloidal suspension.
Mots clés
3D biocompatible matrices, biocompatibility, colloidal particles, implantation, tissue engineering
Référence
ACS Appl Mater Interfaces. 2020 Oct 16;: