Osteogenetic properties of electrospun nanofibrous PCL scaffolds equipped with chitosan-based nanoreservoirs of growth factors.
Fiche publication
Date publication
janvier 2014
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BENKIRANE-JESSEL Nadia, Pr MELY Yves, Dr FIORETTI Florence, Dr RICHERT Ludovic
Tous les auteurs :
Ferrand A, Eap S, Richert L, Lemoine S, Kalaskar D, Demoustier-Champagne S, Atmani H, Mely Y, Fioretti F, Schlatter G, Kuhn L, Ladam G, Benkirane-Jessel N
Lien Pubmed
Résumé
Bioactive implants intended for rapid, robust, and durable bone tissue regeneration are presented. The implants are based on nanofibrous 3D-scaffolds of bioresorbable poly--caprolactone mimicking the fibrillar architecture of bone matrix. Layer-by-layer nanoimmobilization of the growth factor BMP-2 in association with chitosan (CHI) or poly-L-lysine over the nanofibers is described. The osteogenetic potential of the scaffolds coated with layers of CHI and BMP-2 is demonstrated in vitro, and in vivo in mouse calvaria, through enhanced osteopontin gene expression and calcium phosphate biomineralization. The therapeutic strategy described here contributes to the field of regenerative medicine, as it proposes a route toward efficient repair of bone defects at reduced risk and cost level.
Référence
Macromol Biosci. 2014 Jan;14(1):45-55