Characterization of Bone Marrow and Wharton's Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering.
Fiche publication
Date publication
septembre 2020
Journal
Polymers
Auteurs
Membres identifiés du Cancéropôle Est :
Dr VELARD Frédéric, Dr DE ISLA Natalia
Tous les auteurs :
Liu X, Baldit A, de Brosses E, Velard F, Cauchois G, Chen Y, Wang X, de Isla N, Laurent C
Lien Pubmed
Résumé
(1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem cells (Wharton's jelly mesenchymal stromal cells (WJ-MSCs) and bone marrow mesenchymal stromal cells (BM-MSCs)) were assessed. The scaffold configurations consisted of multilayer braids with various number of silk layers ( = 1, 2, 3), and a novel composite scaffold made of a layer of copoly(lactic acid--(e-caprolactone)) (PLCL) embedded between two layers of silk. (3) Results: The insertion of a PLCL layer resulted in a higher porosity and better mechanical behavior compared with pure silk scaffold. The metabolic activities of both WJ-MSCs and BM-MSCs increased from day 1 to day 7 except for the three-layer silk scaffold (S3), probably due to its lower porosity. Collagen I (Col I), collagen III (Col III) and tenascin-c (TNC) were expressed by both MSCs on all scaffolds, and expression of Col I was higher than Col III and TNC. (4) Conclusions: the silk/PLCL composite scaffolds constituted the most suitable tested configuration to support MSCs migration, proliferation and tissue synthesis towards ligament tissue engineering.
Mots clés
PLCL, biocompatibility, braided scaffold, ligament tissue engineering, silk, stromal cells
Référence
Polymers (Basel). 2020 Sep 22;12(9):