Mechanobiologically induced bone-like nodules: Matrix characterization from micro to nanoscale.
Fiche publication
Date publication
juin 2020
Journal
Nanomedicine : nanotechnology, biology, and medicine
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie, Dr TERRYN Christine, Dr KERDJOUDJ Halima
Tous les auteurs :
Rammal H, Dubus M, Bercu NB, Mathieu E, Terryn C, Molinari M, Gangloff SC, Nassif N, Mauprivez C, Quilès F, Kerdjoudj H
Lien Pubmed
Résumé
In bone tissue engineering, stem cells are known to form inhomogeneous bone-like nodules on a micrometric scale. Herein, micro- and nano-infrared (IR) micro-spectroscopies were used to decipher the chemical composition of the bone-like nodule. Histological and immunohistochemical analysesanalyzes revealed a cohesive tissue with bone-markers positive cells surrounded by dense mineralized type-I collagen. Micro-IR gathered complementary information indicating a non-mature collagen at the top and periphery and a mature collagen within the nodule. Atomic force microscopy combined to IR (AFM-IR) analysesanalyzes showed distinct spectra of "cell" and "collagen" rich areas. In contrast to the "cell" area, spectra of "collagen" area revealed the presence of carbohydrate moieties of collagen and/or the presence of glycoproteins. However, it was not possible to determine the collagen maturity, due to strong bands overlapping and/or possible proteins orientation effects. Such findings could help developing protocols to allow a reliable characterization of in vitro generated complex bone tissues.
Mots clés
AFM-IR spectroscopy, Bone-like nodule, Differentiated stem cells, Infrared microscopy, Nano/micro scale
Référence
Nanomedicine. 2020 Jun 29;:102256