Posttranslational modification of Elongation Factor P from Staphylococcus aureus.
Fiche publication
Date publication
mai 2020
Journal
FEBS open bio
Auteurs
Membres identifiés du Cancéropôle Est :
Dr NEGRONI Luc, Dr YUSUPOV Marat, Dr YUSUPOVA Gulnara
Tous les auteurs :
Golubev A, Negroni L, Krasnovid F, Validov S, Yusupova G, Yusupov M, Usachev K
Lien Pubmed
Résumé
Antibiotic resistant Staphylococcus aureus is becoming a major burden on healthcare systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF-P) is a highly conserved elongation protein factor that plays an important role in protein synthesis and bacteria virulence. EF-P undergoes unique posttranslational modifications in a stepwise manner in order to function correctly, but experimental information on EF-P posttranslational modifications is currently lacking for S. aureus. Here, we expressed EF-P in S. aureus to analyze its posttranslational modifications by mass spectrometry, and report experimental proof of 5-aminopentanol modification of S. aureus EF-P.
Mots clés
EF-P, Ribosome, Staphylococcus aureus, posttranslational modifications, stalling
Référence
FEBS Open Bio. 2020 May 20;: