Submicron polycaprolactone particles as a carrier for imaging contrast agent for in vitro applications.
Fiche publication
Date publication
décembre 2015
Journal
Colloids and surfaces. B, Biointerfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HUMBERT Philippe
Tous les auteurs :
Iqbal M, Robin S, Humbert P, Viennet C, Agusti G, Fessi H, Elaissari A
Lien Pubmed
Résumé
Fluorescent materials have recently attracted considerable attention due to their unique properties and high performance as imaging agent in biomedical fields. Different imaging agents have been encapsulated in order to restrict its delivery to a specific area. In this study, a fluorescent contrast agent was encapsulated for in vitro application by polycaprolactone (PCL) polymer. The encapsulation was performed using modified double emulsion solvent evaporation technique with sonication. Fluorescent nanoparticles (20 nm) were incorporated in the inner aqueous phase of double emulsion. A number of samples were fabricated using different concentrations of fluorescent contrast agent. The contrast agent-containing submicron particle was characterized by a zetasizer for average particle size, SEM and TEM for morphology observations and fluorescence spectrophotometer for encapsulation efficiency. Moreover, contrast agent distribution in the PCL matrix was determined by confocal microscopy. The incorporation of contrast agent in different concentrations did not affect the physicochemical properties of PCL particles and the average size of encapsulated particles was found to be in the submicron range.
Mots clés
Contrast Media, administration & dosage, Drug Carriers, Humans, In Vitro Techniques, Microscopy, Confocal, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Polyesters, chemistry
Référence
Colloids Surf B Biointerfaces. 2015 Dec;136:488-95