Reversing charges or how to improve Wharton's jelly mesenchymal stem cells culture on polyelectrolyte multilayer films.
Fiche publication
Date publication
janvier 2013
Journal
Bio-medical materials and engineering
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MENU Patrick, Dr KERDJOUDJ Halima
Tous les auteurs :
Rammal H, Beroud J, Gentils M, Labrude P, Menu P, Kerdjoudj H, Velot E
Lien Pubmed
Résumé
Polyelectrolyte multilayer (PEMs) films made of poly(allylamine hydrochloride) (PAH) as polycation and poly(styrene sulfonate) (PSS) as polyanion, with a PAH ending layer, can be used as a coating in order to improve the anti-thrombogenicity and patency of vascular grafts in vascular engineering field. They induce strong adhesion of mature endothelial cells on glass, expanded polytetrafluoroethylene and cryopreserved arteries. Despite their outstanding effect on mature and progenitor endothelial cells, PEMs ending with PAH showed a poor outcome on Wharton's jelly mesenchymal stem cells (WJ-MSCs) culture.
Mots clés
5'-Nucleotidase, analysis, Antigens, CD, analysis, Antigens, CD34, analysis, Biocompatible Materials, chemistry, Cations, chemistry, Cell Adhesion, Cell Culture Techniques, Cell Proliferation, Cell Shape, Coated Materials, Biocompatible, chemistry, Electrochemistry, Endoglin, Flow Cytometry, GPI-Linked Proteins, analysis, Glass, chemistry, Humans, Leukocyte Common Antigens, analysis, Mesenchymal Stromal Cells, physiology, Phenotype, Polyamines, chemistry, Polymers, chemistry, Polystyrenes, chemistry, Receptors, Cell Surface, analysis, Surface Properties, Thy-1 Antigens, analysis
Référence
Biomed Mater Eng. 2013 ;23(4):299-309