Multiplexed Detection of Cancer Serum Antigens with a Quantum Dot-Based Lab-on-Bead System.
Fiche publication
Date publication
janvier 2020
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr NABIEV Igor
Tous les auteurs :
Tsoy T, Karaulov A, Nabiev I, Sukhanova A
Lien Pubmed
Résumé
A quantum dot (QD)-based lab-on-bead system is a unique tool for multiple analysis of cancer markers in human serum samples by using a flow cytometer. In terms of specificity and sensitivity, this method is comparable with ELISA, the "gold standard" of serological in-clinic detection of single analytes. Fluorescent microspheres encoded with QDs have been used for the quantitative detection of free and total prostate-specific antigen in human serum samples. Developed multiplex assay demonstrates a clear discrimination between serum samples from control subjects and cancer patients. The proposed QD-based method is adaptable and makes it possible to develop numerous clinical tests with decreased duration and cost for early diagnosis of various diseases.
Mots clés
Cancer markers, Flow cytometry, Multiplex analysis, Nanoparticle, Semiconductor quantum dot, Suspension array
Référence
Methods Mol. Biol.. 2020 ;2135:225-236