Embryo Form Project: An original technique for the three-dimensional reconstruction of human embryo morphology.
Fiche publication
Date publication
décembre 2015
Journal
Reproductive toxicology (Elmsford, N.Y.)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr LABROUSSE Marc
Tous les auteurs :
Labrousse M, Micard E, Tonnelet R, Cendre R, Delmas V, Naidich T, Braun M
Lien Pubmed
Résumé
Our current knowledge on the developmental stages of human embryogenesis has derived from limited numbers of classical studies. Computer technology now permits accurate 3D reconstruction of embryo morphology from serial histological sections. We present a successful technique that uses either fresh or preserved serial histological sections to generate highly detailed 3D image reconstructions of very small human embryos. We present the data we obtained from the reconstruction in virtual space of previously sectioned 15 and 22.5mm embryos. Their morphologies were studied using a DICOM viewer which permitted the analysis of any specific structure in any required orientation. To our knowledge, this is the first time human embryos have been reconstructed in this way. We believe that this reconstruction technique could improve our knowledge on embryo morphogenesis, especially if coupled to the study of genes involved in embryonic development. It may also prove to be a useful pedagogical tool.
Mots clés
Embryo, Mammalian, anatomy & histology, Gestational Age, Humans, Imaging, Three-Dimensional, methods, Microtomy, Morphogenesis, Software, Staining and Labeling
Référence
Reprod. Toxicol.. 2015 Dec;58:1-7