Differentiating Fragmentation Pathways of Cholesterol by Two-Dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
Fiche publication
Date publication
décembre 2015
Journal
Journal of the American Society for Mass Spectrometry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DELSUC Marc-André
Tous les auteurs :
van Agthoven MA, Barrow MP, Chiron L, Coutouly MA, Kilgour D, Wootton CA, Wei J, Soulby A, Delsuc MA, Rolando C, O'Connor PB
Lien Pubmed
Résumé
Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry is a data-independent analytical method that records the fragmentation patterns of all the compounds in a sample. This study shows the implementation of atmospheric pressure photoionization with two-dimensional (2D) Fourier transform ion cyclotron resonance mass spectrometry. In the resulting 2D mass spectrum, the fragmentation patterns of the radical and protonated species from cholesterol are differentiated. This study shows the use of fragment ion lines, precursor ion lines, and neutral loss lines in the 2D mass spectrum to determine fragmentation mechanisms of known compounds and to gain information on unknown ion species in the spectrum. In concert with high resolution mass spectrometry, 2D Fourier transform ion cyclotron resonance mass spectrometry can be a useful tool for the structural analysis of small molecules. Graphical Abstract ᅟ.
Mots clés
Atmospheric Pressure, Cholesterol, chemistry, Cyclotrons, Equipment Design, Fourier Analysis, Ions, chemistry, Mass Spectrometry, methods, Photochemical Processes
Référence
J. Am. Soc. Mass Spectrom.. 2015 Dec;26(12):2105-14