Chromatographic Separation Simulation of Metal-Chelating Peptides from Surface Plasmon Resonance Binding Parameters.
Fiche publication
Date publication
mars 2020
Journal
Journal of separation science
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BOSCHI-MULLER Sandrine
Tous les auteurs :
Muhr L, Pontvianne S, Selmeczi K, Paris C, Boschi-Muller S, Canabady-Rochelle L
Lien Pubmed
Résumé
Some metal-chelating peptides have antioxidant properties, with potential nutrition, health and cosmetics applications. This study aimed to simulate their separation on immobilized metal ion affinity chromatography from their affinity constant for immobilised metal ion determined in surface plasmon resonance, both technics being based on peptide-metal ion interactions. In our approach, first, the affinity constant of synthetic peptides was determined by surface plasmon resonance and used as input data to numerically simulate the chromatographic separation with a transport-dispersive model based on Langmuir adsorption isotherm. Then, chromatographic separation was applied on the same peptides to determine their retention time and compare this experimental t with the simulated t obtained from simulation from surface plasmon resonance data. For the investigated peptides, the relative values of t were comparable. Hence, our study demonstrated the pertinence of such numerical simulation correlating immobilised metal ion affinity chromatography and surface plasmon resonance. This article is protected by copyright. All rights reserved.
Mots clés
chelation, immobilized metal ion affinity chromatography, modelling, peptide, surface plasmon resonance
Référence
J Sep Sci. 2020 Mar 3;: