Sodium and Potassium Interactions with Nucleic Acids.
Fiche publication
Date publication
janvier 2016
Journal
Metal ions in life sciences
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ENNIFAR Eric
Tous les auteurs :
Auffinger P, D'Ascenzo L, Ennifar E
Lien Pubmed
Résumé
Metal ions are essential cofactors for the structure and functions of nucleic acids. Yet, the early discovery in the 70s of the crucial role of Mg(2+) in stabilizing tRNA structures has occulted for a long time the importance of monovalent cations. Renewed interest in these ions was brought in the late 90s by the discovery of specific potassium metal ions in the core of a group I intron. Their importance in nucleic acid folding and catalytic activity is now well established. However, detection of K(+) and Na(+) ions is notoriously problematic and the question about their specificity is recurrent. Here we review the different methods that can be used to detect K(+) and Na(+) ions in nucleic acid structures such as X-ray crystallography, nuclear magnetic resonance or molecular dynamics simulations. We also discuss specific versus non-specific binding to different structures through various examples.
Mots clés
Cesium, chemistry, Crystallography, X-Ray, DNA, chemistry, Hydrogen Bonding, Magnetic Resonance Spectroscopy, Models, Molecular, Nucleic Acid Conformation, Potassium, chemistry, RNA, chemistry, Rubidium, chemistry, Sodium, chemistry
Référence
Met Ions Life Sci. 2016 ;16:167-201