Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins.
Fiche publication
Date publication
janvier 2016
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr YUSUPOV Marat, Dr YUSUPOVA Gulnara
Tous les auteurs :
Agalarov S, Yusupov M, Yusupova G
Lien Pubmed
Résumé
In vitro reconstitution systems of ribosomal subunits from free ribosomal RNA and ribosomal proteins are helpful tool for studies on the structure, function and assembly of ribosome. Using this system mutant or modified ribosomal proteins or ribosomal RNA can be incorporated into ribosomal subunits for studying ribosome structure and function. Developing the protocol for reconstitution of 30S subunits from an extreme thermophilic bacterium Thermus thermophilus can be beneficial especially for structural studies, as proteins and nucleic acids from this organism are very stable and crystallize easier than those from mesophilic organisms.
Mots clés
Centrifugation, Density Gradient, Crystallization, Nucleic Acid Conformation, Nucleic Acids, chemistry, Poly U, Polyamines, chemistry, RNA, chemistry, RNA, Bacterial, genetics, RNA, Ribosomal, genetics, RNA, Ribosomal, 16S, chemistry, RNA, Transfer, chemistry, Ribosomal Proteins, chemistry, Ribosome Subunits, Small, Bacterial, chemistry, Ribosomes, chemistry, Thermus thermophilus, chemistry
Référence
Methods Mol. Biol.. 2016 ;1320:303-14