Adenovirus-specific T-cell Subsets in Human Peripheral Blood and After IFN-γ Immunomagnetic Selection.
Fiche publication
Date publication
janvier 2016
Journal
Journal of immunotherapy (Hagerstown, Md. : 1997)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BENSOUSSAN Danièle, Pr DE CARVALHO BITTENCOURT Marcelo, Pr DECOT Véronique, Dr REPPEL Loïc
Tous les auteurs :
Qian C, Wang Y, Cai H, Laroye C, De Carvalho Bittencourt M, Clement L, Stoltz JF, Decot V, Reppel L, Bensoussan D
Lien Pubmed
Résumé
Adoptive antiviral cellular immunotherapy by infusion of virus-specific T cells (VSTs) is becoming an alternative treatment for viral infection after hematopoietic stem cell transplantation. The T memory stem cell (TSCM) subset was recently described as exhibiting self-renewal and multipotency properties which are required for sustained efficacy in vivo. We wondered if such a crucial subset for immunotherapy was present in VSTs. We identified, by flow cytometry, TSCM in adenovirus (ADV)-specific interferon (IFN)-γ+ T cells before and after IFN-γ-based immunomagnetic selection, and analyzed the distribution of the main T-cell subsets in VSTs: naive T cells (TN), TSCM, T central memory cells (TCM), T effector memory cell (TEM), and effector T cells (TEFF). In this study all of the different T-cell subsets were observed in the blood sample from healthy donor ADV-VSTs, both before and after IFN-γ-based immunomagnetic selection. As the IFN-γ-based immunomagnetic selection system sorts mainly the most differentiated T-cell subsets, we observed that TEM was always the major T-cell subset of ADV-specific T cells after immunomagnetic isolation and especially after expansion in vitro. Comparing T-cell subpopulation profiles before and after in vitro expansion, we observed that in vitro cell culture with interleukin-2 resulted in a significant expansion of TN-like, TCM, TEM, and TEFF subsets in CD4IFN-γ T cells and of TCM and TEM subsets only in CD8IFN-γ T cells. We demonstrated the presence of all T-cell subsets in IFN-γ VSTs including the TSCM subpopulation, although this was weakly selected by the IFN-γ-based immunomagnetic selection system.
Mots clés
Adenoviridae, immunology, Adenoviridae Infections, immunology, Antigens, Surface, metabolism, Cell Culture Techniques, Cytotoxicity, Immunologic, Healthy Volunteers, Humans, Immunologic Memory, Immunomagnetic Separation, Immunophenotyping, Immunotherapy, Adoptive, Interferon-gamma, metabolism, Lymphocyte Count, Phenotype, T-Cell Antigen Receptor Specificity, immunology, T-Lymphocyte Subsets, immunology
Référence
J. Immunother.. 2016 Jan;39(1):27-35