In Vitro and In Vivo Activity of Anogeissus leiocarpa Bark Extract and Isolated Metabolites against Toxoplasma gondii.
Fiche publication
Date publication
janvier 2020
Journal
Planta medica
Auteurs
Membres identifiés du Cancéropôle Est :
Dr VELARD Frédéric, Pr VILLENA Isabelle
Tous les auteurs :
Spalenka J, Hubert J, Voutquenne-Nazabadioko L, Escotte-Binet S, Borie N, Velard F, Villena I, Aubert D, Renault JH
Lien Pubmed
Résumé
, belonging to the Apicomplexa phylum, is a cosmopolitan protozoan parasite that affects at least 30% of the world's population. In West Africa, the leaves and bark of the tree species (DC.) Guill. & Perr. are used against zoonosis in traditional medicine and play a key role in controlling diseases induced by Apicomplexans such as malaria. In this study, extracts, fractions, and pure compounds obtained from an ethanol extract of the bark of were evaluated against infection and . The crude bark extract showed significant activity on tachyzoites from the RH strain (IC = 59.30 µg/mL). The crude bark extract without tannins and pure trachelosperogenin E purified by centrifugal partition chromatography showed the highest activity (ICs = 12.83 and 26.63 µg/mL, respectively) with satisfying selectivity indexes of 9.61 and 9.75, respectively. The crude bark extract without tannins and pure trachelosperogenin E were able to significantly inhibit host cell invasion by the parasite , while the crude bark extract without tannins was able to increase mice survival in our murine model of chronic toxoplasmosis. These results provide new biological data for natural compounds that could enhance the current panoply of treatments against toxoplasmosis.
Référence
Planta Med.. 2020 Jan 28;: