Innovative High Gas Pressure Microscopy Chamber Designed for Biological Cell Observation.
Fiche publication
Date publication
février 2016
Journal
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Auteurs
Membres identifiés du Cancéropôle Est :
Pr PERRIER-CORNET Jean-Marie
Tous les auteurs :
Ragon M, Nguyen Thi Minh H, Guyot S, Loison P, Burgaud G, Dupont S, Beney L, Gervais P, Perrier-Cornet JM
Lien Pubmed
Résumé
An original high-pressure microscopy chamber has been designed for real-time visualization of biological cell growth during high isostatic (gas or liquid) pressure treatments up to 200 MPa. This new system is highly flexible allowing cell visualization under a wide range of pressure levels as the thickness and the material of the observation window can be easily adapted. Moreover, the design of the observation area allows different microscope objectives to be used as close as possible to the observation window. This chamber can also be temperature controlled. In this study, the resistance and optical properties of this new high-pressure chamber have been tested and characterized. The use of this new chamber was illustrated by a real-time study of the growth of two different yeast strains - Saccharomyces cerevisiae and Candida viswanathii - under high isostatic gas pressure (30 or 20 MPa, respectively). Using image analysis software, we determined the evolution of the area of colonies as a function of time, and thus calculated colony expansion rates.
Mots clés
Candida, cytology, Cytological Techniques, instrumentation, Gases, Hydrostatic Pressure, Microscopy, instrumentation, Saccharomyces cerevisiae, cytology
Référence
Microsc. Microanal.. 2016 Feb;22(1):63-70