Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation.
Fiche publication
Date publication
février 2016
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Correia CR, Pirraco RP, Cerqueira MT, Marques AP, Reis RL, Mano JF
Lien Pubmed
Résumé
A new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the "stem cell niche", the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources.
Mots clés
Alginates, chemistry, Bone Morphogenetic Protein 2, genetics, Capsules, chemistry, Cell Differentiation, Cells, Cultured, Chitosan, chemistry, Coculture Techniques, Core Binding Factor Alpha 1 Subunit, genetics, Cytokines, analysis, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fluorescent Dyes, chemistry, Glucuronic Acid, chemistry, Hexuronic Acids, chemistry, Humans, Integrin-Binding Sialoprotein, genetics, Microscopy, Electron, Scanning, Microscopy, Fluorescence, Nanostructures, chemistry, Osteoblasts, cytology, Osteogenesis, Osteopontin, metabolism, Platelet Endothelial Cell Adhesion Molecule-1, genetics, Polylysine, chemistry, Real-Time Polymerase Chain Reaction, Vascular Endothelial Growth Factor A, genetics
Référence
Sci Rep. 2016 Feb 24;6:21883