Light-harvesting nanoparticle probes for FRET-based detection of oligonucleotides with single-molecule sensitivity.
Fiche publication
Date publication
janvier 2020
Journal
Angewandte Chemie (International ed. in English)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr KLYMCHENKO Andrey
Tous les auteurs :
Melnychuk N, Egloff S, Runser A, Reisch A, Klymchenko AS
Lien Pubmed
Résumé
Controlling emission of bright luminescent nanoparticles by a single molecular recognition event remains an ultimate challenge in the design of ultrasensitive probes for biomolecules. It would require an efficient Förster resonance energy transfer (FRET) from the nanoparticle to a single acceptor at its surface, which is not realized to date. Here, we developed 20-nm light-harvesting nanoantenna particles, built of a tailor-made hydrophobic charged polymer poly(ethyl methacrylate-co-methacrylic acid), encapsulating ~1000 strongly coupled and highly emissive rhodamine dyes with their bulky counterion. Being 87-fold brighter than quantum dots QDots605 in single-particle microscopy (with 550-nm excitation), these DNA-functionalized nanoparticles exhibit >50% total FRET efficiency to a single hybridized FRET acceptor, a highly photostable carbopyronine dye (ATTO665). The obtained FRET nanoprobes enable single-molecule detection of short DNA and RNA, encoding a cancer marker, and imaging single hybridization events by an epi-fluorescence microscope with ultralow excitation irradiance close to ambient sunlight.
Mots clés
Förster resonance energy transfer, fluorescent nanoparticles, light-harvesting nanoantenna, nucleic acids biosensor, single-molecule detection
Référence
Angew. Chem. Int. Ed. Engl.. 2020 Jan 15;: