Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards.
Fiche publication
Date publication
mars 2016
Journal
Journal of virological methods
Auteurs
Membres identifiés du Cancéropôle Est :
Dr AVEROUS Gerlinde, Pr BALDAUF Jean-Jacques, Dr FENDER Muriel, Pr VELTEN Michel
Tous les auteurs :
Barth H, Morel A, Mougin C, Averous G, Legrain M, Fender M, Risch S, Fafi-Kremer S, Velten M, Oudet P, Baldauf JJ, Stoll-Keller F
Lien Pubmed
Résumé
Biobanking or collection and storage of specimens for future research purposes have become an essential tool in many fields of biomedical research and aims to provide a better understanding of disease mechanisms as well as the identification of disease-specific biomarkers that can navigate in complex diseases. In this study, we assessed the use of Flinders Technology Associates (FTA) cards as a long-term storage device for cervical specimens with suspected human papillomavirus (HPV) infections. HPV detection and genotyping results in liquid-based transport media were compared to HPV results from FTA cards. The overall agreement for the presence of any HPV infection between liquid-based medium and FTA cards stored for 1 year at ambient temperature was 100%. Reproducibility analysis of HPV detection and genotyping from FTA cards demonstrated that FTA cards are a reliable medium to store and preserve viral nucleic acids. Biobanking of cervical cells on FTA cards may provide a key resource for epidemiological and retrospective HPV studies.
Mots clés
Adult, Aged, Cervix Uteri, virology, Cytological Techniques, methods, DNA, Viral, genetics, Female, Genotyping Techniques, methods, Humans, Middle Aged, Papillomaviridae, genetics, Papillomavirus Infections, diagnosis, Preservation, Biological, methods, Reproducibility of Results, Retrospective Studies, Specimen Handling, methods, Virology, methods, Young Adult
Référence
J. Virol. Methods. 2016 Mar;229:60-5