DAZL and CPEB1 regulate mRNA translation synergistically during oocyte maturation.
Fiche publication
Date publication
mars 2016
Journal
Journal of cell science
Auteurs
Membres identifiés du Cancéropôle Est :
Pr VIVILLE Stéphane
Tous les auteurs :
Sousa Martins JP, Liu X, Oke A, Arora R, Franciosi F, Viville S, Laird DJ, Fung JC, Conti M
Lien Pubmed
Résumé
Meiotic progression requires exquisitely coordinated translation of maternal messenger (m)RNA that has accumulated during oocyte growth. A major regulator of this program is the cytoplasmic polyadenylation element binding protein 1 (CPEB1). However, the temporal pattern of translation at different meiotic stages indicates the function of additional RNA binding proteins (RBPs). Here, we report that deleted in azoospermia-like (DAZL) cooperates with CPEB1 to regulate maternal mRNA translation. Using a strategy that monitors ribosome loading onto endogenous mRNAs and a prototypic translation target, we show that ribosome loading is induced in a DAZL- and CPEB1-dependent manner, as the oocyte reenters meiosis. Depletion of the two RBPs from oocytes and mutagenesis of the 3' untranslated regions (UTRs) demonstrate that both RBPs interact with the Tex19.1 3' UTR and cooperate in translation activation of this mRNA. We observed a synergism between DAZL and cytoplasmic polyadenylation elements (CPEs) in the translation pattern of maternal mRNAs when using a genome-wide analysis. Mechanistically, the number of DAZL proteins loaded onto the mRNA and the characteristics of the CPE might define the degree of cooperation between the two RBPs in activating translation and meiotic progression.
Mots clés
Animals, Female, Gene Expression Regulation, Developmental, Male, Meiosis, Mice, Inbred C57BL, Oocytes, cytology, Oogenesis, Protein Biosynthesis, RNA, Messenger, genetics, RNA-Binding Proteins, genetics, Transcription Factors, genetics, mRNA Cleavage and Polyadenylation Factors, genetics
Référence
J. Cell. Sci.. 2016 Mar;129(6):1271-82