Peptide-functionalized ZCIS QDs as fluorescent nanoprobe for targeted HER2-positive breast cancer cells imaging.
Fiche publication
Date publication
avril 2016
Journal
Acta biomaterialia
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SCHNEIDER Raphaël
Tous les auteurs :
Michalska M, Florczak A, Dams-Kozlowska H, Gapinski J, Jurga S, Schneider R
Lien Pubmed
Résumé
In this paper, the synthesis of alloyed CuInZnxS2+x quantum dots (ZCIS QDs), their transfer into aqueous solution via a polymer coating technique, and the use of these nanocrystals to selectively target HER2-positive cells, are reported. By optimizing first the ZnS shell deposition process onto the CuInS2 core, and next the encapsulation of the dots with the amphiphilic poly(maleic anhydride-alt-1-octadecene) (PMAO) polymer, water-dispersible ZCIS QDs were successfully prepared. The nanocrystals with a photoluminescence quantum yield of 35% were purified via centrifugation and ultracentrifugation and high quality nanoparticles with narrow size distributions and surface charges were obtained. After verifying the biocompatibility of PMO-coated ZCIS QDs, we coupled these nanocrystals with the LTVSPWY peptide and demonstrated via MTT assay that both bare and the peptide-linked QDs exhibit low cytotoxicity. The HER2-mediated delivery of the peptide-linked QDs was confirmed by confocal microscopy. This study indicates that as engineered QDs can efficiently be used as fluorescent nanoprobes for selective labelling of HER2-positive SKBR3 cancer cells.
Mots clés
Amino Acid Sequence, Animals, Breast Neoplasms, diagnosis, Cell Death, Diagnostic Imaging, methods, Female, Flow Cytometry, Fluorescence, Humans, Maleic Anhydrides, chemical synthesis, Mice, Mitochondria, metabolism, NIH 3T3 Cells, Nanoparticles, chemistry, Particle Size, Peptides, chemistry, Polymers, chemical synthesis, Quantum Dots, chemistry, Receptor, ErbB-2, metabolism, Sulfides
Référence
Acta Biomater. 2016 Apr;35:293-304