SMART Digest™ compared with pellet digestion for analysis of human immunoglobulin G1 in rat serum by liquid chromatography tandem mass spectrometry.
Fiche publication
Date publication
mai 2016
Journal
Analytical biochemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CIANFERANI Sarah
Tous les auteurs :
Lanshoeft C, Heudi O, Cianférani S
Lien Pubmed
Résumé
The newly developed SMART Digest™ kit was applied for the sample preparation of human immunoglobulin G1 (hIgG1) in rat serum prior to qualitative and quantitative analyses by liquid chromatography tandem mass spectrometry (LC-MS/MS). The sequence coverages obtained for the light and heavy chains of hIgG1A were 50 and 76%, respectively. The calibration curve was linear from 1.00 to 1000 μg/ml for three of four generic peptides. Overall, the SMART Digest™ kit resulted in similar quantitative data (linearity, sensitivity, accuracy, and precision) compared with the pellet digestion protocol. However, the SMART Digest™ required only 2 h of sample preparation with fewer reagents.
Mots clés
Amino Acid Sequence, Animals, Chromatography, Liquid, Humans, Immunoglobulin G, analysis, Limit of Detection, Rats, Tandem Mass Spectrometry
Référence
Anal. Biochem.. 2016 May;501:23-5