Time-resolved Fluorescence and Generalized Polarization: Innovative tools to assess bull sperm membrane dynamics during slow freezing.
Fiche publication
Date publication
octobre 2019
Journal
Cryobiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr PERRIER-CORNET Jean-Marie
Tous les auteurs :
Bechoua S, Winckler P, Jossier A, Peltier C, Delize F, Devaux N, Perrier Cornet JM, Simonin H
Lien Pubmed
Résumé
During slow freezing, spermatozoa undergo membrane alterations that compromise their ability of fertilizing. These alterations are cause either by cold shock or by the use of cryoprotectants known to be cytotoxic. However, little is known about the membrane changes that occurred during freezing. Here, we combined Generalized Polarization (GP), Time-resolved Fluorescence and laurdan fluorescence properties to investigate the changes in membrane fluidity and dynamics during slow freezing of bull sperm. We successfully demonstrated that laurdan may be distributed in three different local environments that correspond to different membrane lipid composition. These environments wont behave the same way when the cells will be subjected to either a chemical treatment (adding the cryoprotectants) or a physical treatment (freezing).
Mots clés
Bull sperm, GP, Membrane, Slow freezing, Time-resolved fluorescence
Référence
Cryobiology. 2019 Oct 30;: