Endophytic fungi associated with Sudanese medicinal plants show cytotoxic and antibiotic potential.
Fiche publication
Date publication
juin 2016
Journal
FEMS microbiology letters
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SCHOHN Hervé
Tous les auteurs :
Khiralla A, Mohamed IE, Tzanova T, Schohn H, Slezack-Deschaumes S, Hehn A, André P, Carre G, Spina R, Lobstein A, Yagi S, Laurain-Mattar D
Lien Pubmed
Résumé
In this study, we isolated 15 endophytic fungi from five Sudanese medicinal plants. Each fungal endophytic strain was identified by sequencing of internal transcribed spacer (ITS) regions of rDNA. Ethyl acetate extracts were prepared from each endophyte cultivated in vitro and tested for their respective antibacterial activities and antiproliferative activities against human cancer cells. Antibacterial screening was carried out against two bacterial strains: Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus, by the broth dilution method. Cell viability was evaluated by the MTT procedure after exposure of MCF7 breast cancer cells and HT29 or HCT116 human colon adenocarcinoma cells to each endophytic extract. Of interest, Byssochlamys spectabilis isolated from Euphorbia prostata showed cytotoxicity (IC50 = 1.51 ± 0.2 μg mL(-1)) against MCF7 cells, but had a low effect against HT29 or HCT116 cells (IC50 > 20 μg mL(-1)). Cladosporium cladosporioides 2, isolated from Vernonia amygdalina leaves, showed antiproliferative activities against MCF7 cells (IC50 = 10.5 ± 1.5 μg mL(-1)) only. On the other hand, B. spectabilis and Alternaria sp. extract had antibacterial activities against the S. aureus strain. The findings of this work revealed that endophytic fungi associated with medicinal plants from Sudan could be considered as an attractive source of new therapeutic compounds.
Mots clés
Sudanese medicinal plants, antibacterial activity, endophytic fungi, human cell viability cancer cell line
Référence
FEMS Microbiol. Lett.. 2016 06;363(11):