Engineering Enriched Microenvironments with Gradients of Platelet Lysate in Hydrogel Fibers.
Fiche publication
Date publication
juin 2016
Journal
Biomacromolecules
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Santo VE, Babo P, Amador M, Correia C, Cunha B, Coutinho DF, Neves NM, Mano JF, Reis RL, Gomes ME
Lien Pubmed
Résumé
Gradients of physical and chemical cues are characteristic of specific tissue microenvironments and contribute toward morphogenesis and tissue regeneration upon injury. Recent advances on microfluidics and hydrogel manipulation raised the possibility of generating biomimetic biomaterials enriched with bioactive factors and encapsulating cells following designs specifically tailored for a target application. The novelty of this work relies on the combination of methacrylated gellan gum (MeGG) with platelet lysate (PL), aiming to generate novel advanced 3D PL-enriched photo-cross-linkable hydrogels and overcoming the lack of adhesion sites provided by the native MeGG hydrogels. This combination takes advantage of the availability, enriched growth factor composition, and potential autologous application of PL while simultaneously preserving the ability provided by MeGG to tailor mechanical properties, protein release kinetics, and shape of the construct according to the desired goal. Incorporation of PL in the hydrogels significantly improved cellular adhesion and viability in the constructs. The use of microfluidic tools allowed the design of a fiber-like hydrogel incorporating a gradient of PL along the length of the fiber. These spatial protein gradients led to the viability and cell number gradients caused by maintenance of human umbilical vein endothelial cells (HUVECs) survival in the fibers toward the PL-enriched sections in comparison with the nonloaded MeGG sections of the fibers. Altogether, we propose a proof of concept strategy to design a PL gradient biomaterial with potential in tissue engineering approaches and analysis of cell-microenvironment interactions.
Mots clés
Acrolein, analogs & derivatives, Adipose Tissue, cytology, Biomimetic Materials, chemical synthesis, Blood Platelets, chemistry, Cell Adhesion, Cell Survival, Cellular Microenvironment, Human Umbilical Vein Endothelial Cells, Humans, Hydrogels, chemical synthesis, Microfluidics, Polysaccharides, Bacterial, chemistry, Stem Cells, cytology, Surface Properties, Tissue Engineering, Tissue Scaffolds, chemistry
Référence
Biomacromolecules. 2016 06 13;17(6):1985-97