From non-covalent binding to irreversible DNA lesions: nile blue and nile red as photosensitizing agents.
Fiche publication
Date publication
juin 2016
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GRANDEMANGE Stéphanie
Tous les auteurs :
Gattuso H, Besancenot V, Grandemange S, Marazzi M, Monari A
Lien Pubmed
Résumé
We report a molecular modeling study, coupled with spectroscopy experiments, on the behavior of two well known organic dyes, nile blue and nile red, when interacting with B-DNA. In particular, we evidence the presence of two competitive binding modes, for both drugs. However their subsequent photophysical behavior is different and only nile blue is able to induce DNA photosensitization via an electron transfer mechanism. Most notably, even in the case of nile blue, its sensitization capabilities strongly depend on the environment resulting in a single active binding mode: the minor groove. Fluorescence spectroscopy confirms the presence of competitive interaction modes for both sensitizers, while the sensitization via electron transfer, is possible only in the case of nile blue.
Mots clés
Binding Sites, Binding, Competitive, Computer Simulation, DNA, B-Form, chemistry, Electron Transport, Fluorescent Dyes, chemistry, Models, Molecular, Nucleic Acid Conformation, Oxazines, chemistry, Photochemical Processes, Photosensitizing Agents, chemistry, Polydeoxyribonucleotides, chemistry, Spectrometry, Fluorescence, Spectrophotometry
Référence
Sci Rep. 2016 06 22;6:28480