Dissecting the role of H3K64me3 in mouse pericentromeric heterochromatin.
Fiche publication
Date publication
janvier 2013
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DAUJAT Sylvain
Tous les auteurs :
Lange UC, Siebert S, Wossidlo M, Weiss T, Ziegler-Birling C, Walter J, Torres-Padilla ME, Daujat S, Schneider R
Lien Pubmed
Résumé
To ensure genome stability, pericentromeric regions are compacted in a dense heterochromatic structure through a combination of specific 'epigenetic' factors and modifications. A cascadal pathway is responsible for establishing pericentromeric chromatin involving chromatin modifiers and 'readers', such as H3K9 histone methyltransferases (Suv)39h and heterochromatin protein 1. Here we define how H3K64me3 on the lateral surface of the histone octamer integrates within the heterochromatinization cascade. Our data suggest that enrichment of H3K64me3 at pericentromeric chromatin foci is dependent on H3K9me3 but independent of a number of central factors such as heterochromatin protein 1, DNA methyltransferases and Suv4-20h histone methyltransferases. Our results support a model in which pericentromeric heterochromatin foci are formed along distinct pathways upon H3K9 trimethylation, involving H3K64me3 to potentially stabilize DNA-histone interactions, as well as sequential recruitment of repressive histone tail and DNA modifications. We hence suggest that multiple mechanisms ensure heterochromatin integrity at pericentromeres, with H3K64me3 as an important factor.
Mots clés
Animals, Centromere, metabolism, Chromosomal Proteins, Non-Histone, metabolism, DNA Methylation, genetics, Fluorescent Antibody Technique, Heterochromatin, metabolism, Histone-Lysine N-Methyltransferase, metabolism, Histones, metabolism, Humans, Lysine, metabolism, Mice, NIH 3T3 Cells, Zygote, metabolism
Référence
Nat Commun. 2013 ;4:2233