Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots.
Fiche publication
Date publication
mars 2018
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Pr NABIEV Igor
Tous les auteurs :
Ramos-Gomes F, Bode J, Sukhanova A, Bozrova SV, Saccomano M, Mitkovski M, Krueger JE, Wege AK, Stuehmer W, Samokhvalov PS, Baty D, Chames P, Nabiev I, Alves F
Lien Pubmed
Résumé
Early detection of malignant tumours and, especially, micrometastases and disseminated tumour cells is still a challenge. In order to implement highly sensitive diagnostic tools we demonstrate the use of nanoprobes engineered from nanobodies (single-domain antibodies, sdAbs) and fluorescent quantum dots (QDs) for single- and two-photon detection and imaging of human micrometastases and disseminated tumour cells in ex vivo biological samples of breast and pancreatic metastatic tumour mouse models expressing human epidermal growth factor receptor 2 (HER2) or carcinoembryonic antigen (CEA). By staining thin (5-10 µm) paraffin and thick (50 µm) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Compared to conventional fluorescently labelled antibodies the sdAb-HER2-QD and sdAb-CEA-QD nanoprobes are superior in detecting micrometastases in tissue sections by lower photobleaching and higher brightness of fluorescence signals ensuring much better discrimination of positive signals versus background. Very high two-photon absorption cross-sections of QDs and small size of the nanoprobes ensure efficient imaging of thick tissue sections unattainable with conventional fluorescent probes. The nanobody-QD probes will help to improve early cancer diagnosis and prognosis of progression by assessing metastasis.
Mots clés
Animals, Breast Neoplasms, metabolism, Carcinoembryonic Antigen, immunology, Cell Line, Tumor, Female, Fluorescent Dyes, chemistry, Humans, Mice, Mice, Nude, Microscopy, Confocal, Microscopy, Fluorescence, Multiphoton, Neoplasm Micrometastasis, Quantum Dots, chemistry, Receptor, ErbB-2, immunology, Single-Domain Antibodies, chemistry, Transplantation, Heterologous
Référence
Sci Rep. 2018 03 15;8(1):4595