o-Nitrobenzyl photoremovable groups with fluorescence uncaging reporting properties.
Fiche publication
Date publication
août 2018
Journal
Organic & biomolecular chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr SPECHT Alexandre
Tous les auteurs :
Abou Nakad E, Bolze F, Specht A
Lien Pubmed
Résumé
o-Nitrobenzyl (o-NB) derivatives are the most widely applied photoremovable groups for the study of dynamic biological processes. By introducing different substituents to the benzylic position we were able to generate a fluorescence signal upon irradiation. This signal originates from the formation of a nitrosoketone by-product able to achieve a keto-enol tautomerism leading to pi-conjugated α-hydroxystilbene derivatives. These o-NB caging groups can be used to directly monitor the uncaging event by the release of a detectable fluorescent side-product.
Référence
Org. Biomol. Chem.. 2018 08 22;16(33):6115-6122