Molecular characterization of isolates of waterborne Cryptosporidium spp. collected during an outbreak of gastroenteritis in South Burgundy, France.
Fiche publication
Date publication
juin 2003
Journal
Journal of clinical microbiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BONNIN Alain, Pr KOHLI Evelyne
Tous les auteurs :
Dalle F, Roz P, Dautin G, Di-Palma M, Kohli E, Sire-Bidault C, Fleischmann MG, Gallay A, Carbonel S, Bon F, Tillier C, Beaudeau P, Bonnin A
Lien Pubmed
Résumé
In September 2001, a waterborne outbreak of gastroenteritis occurred in eastern France. Of 31 fecal samples from symptomatic individuals, 19 tested positive for Cryptosporidium with two PCRs targeting the Hsp70 and the 18S rRNA genes of CRYPTOSPORIDIUM: Sequencing of the PCR fragments produced sequences identical to that of Cryptosporidium parvum genotype 1.
Mots clés
Animals, Base Sequence, Cryptosporidiosis, epidemiology, Cryptosporidium parvum, classification, DNA, Ribosomal, analysis, Disease Outbreaks, Feces, parasitology, France, Gastroenteritis, epidemiology, HSP70 Heat-Shock Proteins, genetics, Humans, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Ribosomal, 18S, genetics, Sequence Analysis, DNA, Water, parasitology
Référence
J. Clin. Microbiol.. 2003 Jun;41(6):2690-3