Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling.
Fiche publication
Date publication
août 2016
Journal
Retrovirology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves
Tous les auteurs :
Mekdad HE, Boutant E, Karnib H, Biedma ME, Sharma KK, Malytska I, Laumond G, Roy M, Réal E, Paillart JC, Moog C, Darlix JL, Mély Y, de Rocquigny H
Lien Pubmed
Résumé
In HIV-1 infected cells, the integrated viral DNA is transcribed by the host cell machinery to generate the full length HIV-1 RNA (FL RNA) that serves as mRNA encoding for the Gag and GagPol precursors. Virion formation is orchestrated by Gag, and the current view is that a specific interaction between newly made Gag molecules and FL RNA initiates the process. This in turn would cause FL RNA dimerization by the NC domain of Gag (GagNC). However the RNA chaperoning activity of unprocessed Gag is low as compared to the mature NC protein. This prompted us to search for GagNC co-factors.
Référence
Retrovirology. 2016 Aug;13(1):54