Shrinkage of freeze-dried cryosections of cells: Investigations by EFTEM and cryo-CLEM.
Fiche publication
Date publication
septembre 2016
Journal
Micron (Oxford, England : 1993)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MICHEL Jean, Pr PLOTON Dominique
Tous les auteurs :
Casanova G, Nolin F, Wortham L, Ploton D, Banchet V, Michel J
Lien Pubmed
Résumé
Freeze-drying of cryosections of cells or tissues is considered to be the most efficient preparation for microanalysis purpose related to transmission electron microscopy. It allows the measurements of ions and water contents at the ultrastructural level. However an important drawback is associated to freeze-drying: the shrinkage of the cryosections. The aim of this paper is the investigation of this phenomenon by means of three different methods applied to both hydrated and dehydrated cryosections: direct distance measurements on fiducial points, thickness measurements by energy filtered transmission microscopy (EFTEM) and cryo-correlative light electron microscopy (cryo-CLEM). Measurements in our experimental conditions reveal a lateral shrinkage around 10% but the most important result concerns the lack of differential shrinkage between most of the cellular compartments.
Référence
Micron. 2016 Sep;88:77-83