Competitive adsorption of human immunoglobulin G and albumin: consequences for structure and reactivity of the adsorbed layer.
Fiche publication
Date publication
octobre 1992
Journal
Proceedings of the National Academy of Sciences of the United States of America
Auteurs
Membres identifiés du Cancéropôle Est :
Dr VOEGEL Jean-Claude, Pr SCHAAF Pierre
Tous les auteurs :
Lutanie E, Voegel JC, Schaaf P, Freund M, Cazenave JP, Schmitt A
Lien Pubmed
Résumé
The affinity of polyclonal anti-IgG for human IgG adsorbed on silica surfaces was investigated by two complementary techniques, scanning angle reflectometry and 125I radiotracing. Special attention was paid to compare the reactivity of IgG adsorbed directly or by exchange with already adsorbed albumin. In particular it was shown that (i) in the first case (direct adsorption) the reaction between anti-IgG and adsorbed IgG was in the ratio 1:1 and (ii) in the second case (adsorption by exchange) there was no reaction.
Mots clés
Adsorption, Albumins, chemistry, Animals, Chickens, Humans, Immunoglobulin G, chemistry, Immunoglobulins, chemistry, In Vitro Techniques, Silicon Dioxide, chemistry
Référence
Proc. Natl. Acad. Sci. U.S.A.. 1992 Oct;89(20):9890-4