Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans.
Fiche publication
Date publication
octobre 2016
Journal
Genetics
Auteurs
Membres identifiés du Cancéropôle Est :
Dr JARRIAULT Sophie
Tous les auteurs :
Doitsidou M, Jarriault S, Poole RJ
Lien Pubmed
Résumé
The use of next-generation sequencing (NGS) has revolutionized the way phenotypic traits are assigned to genes. In this review, we describe NGS-based methods for mapping a mutation and identifying its molecular identity, with an emphasis on applications in Caenorhabditis elegans In addition to an overview of the general principles and concepts, we discuss the main methods, provide practical and conceptual pointers, and guide the reader in the types of bioinformatics analyses that are required. Owing to the speed and the plummeting costs of NGS-based methods, mapping and cloning a mutation of interest has become straightforward, quick, and relatively easy. Removing this bottleneck previously associated with forward genetic screens has significantly advanced the use of genetics to probe fundamental biological processes in an unbiased manner.
Référence
Genetics. 2016 Oct;204(2):451-474