"All in All It's Just Another Brick in the Wall" Cooperative Effects of Cytosine Methylation on DNA Structure and Dynamics.
Fiche publication
Date publication
juillet 2019
Journal
The journal of physical chemistry. B
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GRUEZ Arnaud, Pr GRANDEMANGE Stéphanie
Tous les auteurs :
Hognon C, Besancenot V, Gruez A, Grandemange S, Monari A
Lien Pubmed
Résumé
The behavior of the structural parameters of DNA considering different level of methylation in CpG islands is studied by means of full-atom molecular dynamics simulations and electronic circular dichroism, both in an artificial model system and in a gene promoter sequence. It is demonstrated that methylation although intrinsically brings quite local perturbations may, if its level is high enough, induce cooperative effects that strongly modify the DNA backbone torsional parameters altering the helicity as compared to the non-methylated case. Since methylation of CpG island is correlated with the regu-lation of gene expression, understanding the struc-tural modifications induced in DNA is crucial to char-acterize all the fine equilibria into play in epigenetics phenomena.
Référence
J Phys Chem B. 2019 Jul 31;: