Grape seed and skin extract reduces pancreas lipotoxicity, oxidative stress and inflammation in high fat diet fed rats.
Fiche publication
Date publication
décembre 2016
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Auteurs
Membres identifiés du Cancéropôle Est :
Pr KAHN Naim, Dr HICHAMI Aziz
Tous les auteurs :
Aloui F, Charradi K, Hichami A, Subramaniam S, Khan NA, Limam F, Aouani E
Lien Pubmed
Résumé
Obesity is related to an elevated risk of diabetes and the mechanisms whereby fat adversely affects the pancreas are poorly understood. We studied the effect of a high fat diet (HFD) on pancreas steatosis, oxidative stress and inflammation as well as the putative protection afforded by grape seed and skin extract (GSSE). HFD induced body weight gain, without affecting insulinemia, nor glycemia and dropped adiponectemia. HFD also provoked the ectopic deposition of cholesterol and triglyceride, and an oxidative stress characterized by increased lipoperoxidation and carbonylation, inhibition of antioxidant enzyme activities such as CAT, GPx and SOD, depletion of zinc and a concomitant increase in calcium and HO. HFD induced pro-inflammatory chemokines mRNA as RANTES and MCP1 as well as cytokines expression as TNFα, IL6 and IL1β. Importantly GSSE counteracted all the deleterious effects of HFD on pancreas in vivo i-e lipotoxicity, oxidative stress and inflammation. In conclusion, GSSE could find potential applications in fat-induced pancreas lipotoxicity and dysfunction.
Mots clés
Grape seed and skin extract, Inflammation, Obesity, Oxidative stress, Pancreas
Référence
Biomed. Pharmacother.. 2016 Dec;84:2020-2028