Physicochemical characteristics that affect carbon dot safety: lessons from a comprehensive study on a nanoparticle library.
Fiche publication
Date publication
juillet 2019
Journal
International journal of pharmaceutics
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LEBEAU Luc, Pr PONS Françoise
Tous les auteurs :
Fan J, Claudel M, Ronzani C, Arezki Y, Lebeau L, Pons F
Lien Pubmed
Résumé
Carbon dots (CDs) are emerging nanomaterial in medicine and pharmacy. To explore the impact of physicochemical characteristics on their safety, we synthesized a library of 35 CDs exhibiting different size, charge, chemical composition and surface coating, using various starting materials (carbon source and passivation reagent) and carbonization procedures. The 35 CDs triggered different levels of viability loss when incubated with human macrophages at 3-200 µg/mL for 24 h. The smaller NPs (10-20 nm) were more toxic that larger ones (40-100 nm), whereas NPs that aggregated in culture medium were more toxic than dispersed ones. A positive correlation was found between CD charge or nitrogen content and toxicity. Furthermore, a greater toxicity was observed for CDs prepared from high molecular weight polyamines, suggesting a role of the CD global density of positive charges, rather than the charge at the CD surface, in the CD toxicity. At last, PEG decoration decreased the toxicity of cationic NPs. In conclusion, the size, aggregation in culture medium, charge, nitrogen content, nature of the passivation agent and synthesis procedure were found to influence CD toxicity, making it difficult to predict CD safety from a single characteristic.
Mots clés
carbon dot, charge, nanoparticle, size, surface chemistry, toxicity
Référence
Int J Pharm. 2019 Jul 16;:118521