Transforming growth factor-beta 1 or ascorbic acid are able to differentiate Wharton's jelly mesenchymal stem cells towards a smooth muscle phenotype.
Fiche publication
Date publication
janvier 2017
Journal
Bio-medical materials and engineering
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MENU Patrick
Tous les auteurs :
Mesure B, Huber-Villaume S, Menu P, Velot É
Lien Pubmed
Résumé
Wharton's jelly mesenchymal stem cells (WJ-MSCs) are widely used in tissue engineering. In vascular engineering, the ability to obtain a vessel replacement with contractile smooth muscle cells (SMC) is a key factor. In this work, we demonstrated that WJ-MSCs differentiate towards a SMC phenotype with various stimulations in vitro and that the modification of redox state could be involved. WJ-MSCs were isolated from umbilical cords. After their expansion, the cells were stimulated with ascorbic acid (AA, 300 μM) or transforming growth factor (TGF)-β1 (1 to 5 ng/mL). SMC markers were analyzed by Western blot. Modification of redox state was evaluated by reactive oxygen species (ROS) production and glutathione (GSH) content measurements. TGF-β1 or AA-stimulated WJ-MSCs express early and intermediate SMC markers. TGF-β1 (5 ng/mL) modifies the redox state by a ROS production and a GSH content drop, while AA has no effect. This work showed that TGF-β1 and AA are effective SMC phenotype inducers to differentiate WJ-MSCs.
Mots clés
Ascorbic Acid, metabolism, Blood Vessel Prosthesis, Cell Differentiation, Cells, Cultured, Humans, Mesenchymal Stromal Cells, cytology, Muscle Development, Myocytes, Smooth Muscle, cytology, Oxidative Stress, Tissue Engineering, Transforming Growth Factor beta1, metabolism, Wharton Jelly, cytology
Référence
Biomed Mater Eng. 2017 ;28(s1):S101-S105