Permanently Hypoxic Cell Culture Yields Rat Bone Marrow Mesenchymal Cells with Higher Therapeutic Potential in the Treatment of Chronic Myocardial Infarction.
Fiche publication
Date publication
janvier 2017
Journal
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MARIE Pierre-Yves, Pr GAUCHOTTE Guillaume
Tous les auteurs :
Liu Y, Yang X, Maureira P, Falanga A, Marie V, Gauchotte G, Poussier S, Groubatch F, Marie PY, Tran N
Lien Pubmed
Résumé
The mismatch between traditional in vitro cell culture conditions and targeted chronic hypoxic myocardial tissue could potentially hamper the therapeutic effects of implanted bone marrow mesenchymal stem cells (BMSCs). This study sought to address (i) the extent of change to BMSC biological characteristics in different in vitro culture conditions and (ii) the effectiveness of permanent hypoxic culture for cell therapy in treating chronic myocardial infarction (MI) in rats.
Mots clés
Animals, Bone Marrow Cells, cytology, Cell Differentiation, Cell Hypoxia, Cell Proliferation, Cells, Cultured, Cytokines, metabolism, Fluorodeoxyglucose F18, chemistry, Heart, diagnostic imaging, Hemodynamics, Inflammation, Mesenchymal Stem Cell Transplantation, Mesenchymal Stromal Cells, cytology, Myocardial Infarction, pathology, Myocardium, metabolism, Neovascularization, Physiologic, Phenotype, Positron-Emission Tomography, Rats, Rats, Wistar
Référence
Cell. Physiol. Biochem.. 2017 ;44(3):1064-1077