New tools for non-invasive exploration of collagen network in cartilaginous tissue-engineered substitute.
Fiche publication
Date publication
janvier 2017
Journal
Bio-medical materials and engineering
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DUMAS Dominique, Pr GILLET Pierre, Pr MAINARD Didier
Tous les auteurs :
Henrionnet C, Dumas D, Hupont S, Stoltz JF, Mainard D, Gillet P, Pinzano A
Lien Pubmed
Résumé
In tissue engineering approaches, the quality of substitutes is a key element to determine its ability to treat cartilage defects. However, in clinical practice, the evaluation of tissue-engineered cartilage substitute quality is not possible due to the invasiveness of the standard procedure, which is to date histology. The aim of this work was to validate a new innovative system performed from two-photon excitation laser adapted to an optical macroscope to evaluate at macroscopic scale the collagen network in cartilage tissue-engineered substitutes in confrontation with gold standard histologic techniques or immunohistochemistry to visualize type II collagen. This system permitted to differentiate the quality of collagen network between ITS and TGF-β1 treatments. Multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) at macroscopical scale represent an innovative and non-invasive technique to monitor the quality of collagen network in cartilage tissue-engineered substitutes before in vivo implantation.
Référence
Biomed Mater Eng. 2017 ;28(s1):S229-S235