Differential expression of peroxisome proliferator-activated receptors (PPARs) in the developing human fetal digestive tract.
Fiche publication
Date publication
mai 2000
Journal
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Auteurs
Membres identifiés du Cancéropôle Est :
Pr COLLET Pierre, Pr SCHOHN Hervé
Tous les auteurs :
Huin C, Corriveau L, Bianchi A, Keller JM, Collet P, Krémarik-Bouillaud P, Domenjoud L, Bécuwe P, Schohn H, Ménard D, Dauça M
Lien Pubmed
Résumé
We investigated the spatiotemporal distributions of the different peroxisome proliferator-activated receptor (PPAR) isotypes (alpha, beta, and gamma) during development (Week 7 to Week 22 of gestation) of the human fetal digestive tract by immunohistochemistry using specific polyclonal antibodies. The PPAR subtypes, including PPARgamma, are expressed as early as 7 weeks of development in cell types of endodermal and mesodermal origin. The presence of PPARgamma was also found by Western blotting and nuclease-S1 protection assay, confirming that this subtype is not adipocyte-specific. PPARalpha, PPARbeta, and PPARgamma exhibit different patterns of expression during morphogenesis of the digestive tract. Whatever the stage and the gut region (except the stomach) examined, PPARgamma is expressed at a high level, suggesting some fundamental role for this receptor in development and/or physiology of the human digestive tract.
Mots clés
Antibody Specificity, Blotting, Western, Cell Differentiation, Cell Nucleus, metabolism, Colon, cytology, Cytoplasm, metabolism, Digestive System, cytology, Esophagus, cytology, Humans, Intestine, Small, cytology, Receptors, Cytoplasmic and Nuclear, biosynthesis, Stomach, cytology, Transcription Factors, biosynthesis
Référence
J. Histochem. Cytochem.. 2000 May;48(5):603-11