Induction of the expression of the peroxisome proliferator-activated receptor alpha (PPARalpha) by clofibrate in jerboa tissues.
Fiche publication
Date publication
juin 2003
Journal
Microscopy research and technique
Auteurs
Membres identifiés du Cancéropôle Est :
Pr COLLET Pierre, Pr SCHOHN Hervé
Tous les auteurs :
Amsaguine-Safir S, Bianchi A, Collet P, Huin-Schohn C, Jeanvoine A, Bécuwe P, Krémarik-Bouillaud P, Domenjoud L, Keller JM, Schohn H, Dauça M
Lien Pubmed
Résumé
Peroxisome proliferator-activated receptor alpha (PPARalpha) is a member of the nuclear hormone receptor superfamily that can be activated by natural fatty acids and various xenobiotics, including clofibrate. This transcription factor primarily regulates genes involved in lipid metabolism and homeostasis. We present the expression pattern of the PPARalpha subtype in the adult jerboa Jaculus orientalis, determined by RT-PCR and Western blotting using specific probes and a polyclonal antibody for PPARalpha, respectively. PPARalpha is highly expressed in liver and kidney, and to a lesser extent in duodenum and colon. PPARalpha expression is increased at the mRNA and protein levels in liver and duodenum of jerboa treated for 2 weeks with the peroxisome proliferator (PP) clofibrate. The induction is tissue-specific as no significant changes are observed in kidney and colon. The present data indicate that the PP-induced PPARalpha gene expression is not dependent on the PPARalpha content in target cells.
Mots clés
Animals, Clofibrate, pharmacology, Gene Expression Regulation, drug effects, Intestinal Mucosa, metabolism, Kidney, metabolism, Liver, metabolism, Peroxisome Proliferators, pharmacology, Receptors, Cytoplasmic and Nuclear, genetics, Reverse Transcriptase Polymerase Chain Reaction, Rodentia, metabolism, Transcription Factors, genetics
Référence
Microsc. Res. Tech.. 2003 Jun;61(2):185-90