BetaHPV E6 and E7 colocalize with NuMa in dividing keratinocytes.
Fiche publication
Date publication
juillet 2019
Journal
Virus genes
Auteurs
Membres identifiés du Cancéropôle Est :
Pr AUBIN François
Tous les auteurs :
Oswald E, Kirschberg M, Aubin F, Alonso A, Hufbauer M, Akgül B, Auvinen E
Lien Pubmed
Résumé
Human papillomaviruses (HPVs) of genus betapapillomavirus (betaHPV) are implicated in skin carcinogenesis, but their exact role in keratinocyte transformation is poorly understood. We show an interaction of HPV5 and HPV8 oncoproteins E6 and E7 with the nuclear mitotic apparatus protein 1 (NuMA). Binding of E6 or E7 to NuMA induces little aneuploidy, cell cycle alterations, or aberrant centrosomes. Intracellular localization of NuMA is not altered by E6 and E7 expression in 2D cultures. However, the localization profile is predominantly cytoplasmic in 3D organotypic skin models. Both viral proteins colocalize with NuMA in interphase cells, while only E7 colocalizes with NuMA in mitotic cells. Intriguingly, a small subset of cells shows E7 at only one spindle pole, whereas NuMA is present at both poles. This dissimilar distribution of E7 at the spindle poles may alter cell differentiation, which may in turn be relevant for betaHPV-induced skin carcinogenesis.
Mots clés
Betapapillomavirus, E6E7 oncoproteins, Human papillomavirus, Nuclear mitotic apparatus protein 1
Référence
Virus Genes. 2019 Jul 9;: