Differential expression of P450 aromatase during gonadal sex differentiation and sex reversal of the newt Pleurodeles waltl.
Fiche publication
Date publication
janvier 2003
Journal
The Journal of steroid biochemistry and molecular biology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr FLAMENT Stéphane, Dr GRILLIER-VUISSOZ Isabelle, Dr KUNTZ Sandra
Tous les auteurs :
Kuntz S, Chesnel A, Duterque-Coquillaud M, Grillier-Vuissoz I, Callier M, Dournon C, Flament S, Chardard D
Lien Pubmed
Résumé
A better understanding of vertebrate sexual differentiation could be provided by a study of models in which genetic sex determination (GSD) of gonads can be reversed by temperature. In the newt Pleurodeles waltl, a P450 aromatase cDNA was isolated from adult gonads, and the nucleotide or deduced amino acid sequences showed a high level of identity with various vertebrate species. In adults, aromatase expression was found in gonads and brain. In developing gonads, the expression was found to fit with the thermo-sensitive period (TSP) and was detected in both ZZ and ZW larvae, as well as in ZW submitted during the whole TSP to a masculinizing temperature. In the latter individuals, in situ hybridization and semi quantitative RT-PCR showed that, at the end of TSP, aromatase expression was at the same level than in normal ZZ larvae and was significantly lower than in normal ZW ones. Furthermore, temperature-induced down regulation did not occur when heating was performed at the end of TSP. Our results confirm the importance of aromatase regulation in female versus male differentiation and demonstrate that a down regulation of aromatase expression is involved in the process of sex reversal.
Mots clés
Amino Acid Sequence, Animals, Aromatase, biosynthesis, Base Sequence, DNA, Complementary, metabolism, Disorders of Sex Development, Down-Regulation, Female, In Situ Hybridization, Male, Molecular Sequence Data, Pleurodeles, RNA, metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Sex Differentiation, Temperature, Tissue Distribution
Référence
J. Steroid Biochem. Mol. Biol.. 2003 Jan;84(1):89-100