Drosophila C virus systemic infection leads to intestinal obstruction.
Fiche publication
Date publication
décembre 2014
Journal
Journal of virology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr IMLER Jean-Luc, Dr MEIGNIN Carine, Dr DAEFFLER Laurent
Tous les auteurs :
Chtarbanova S, Lamiable O, Lee KZ, Galiana D, Troxler L, Meignin C, Hetru C, Hoffmann JA, Daeffler L, Imler JL
Lien Pubmed
Résumé
Drosophila C virus (DCV) is a positive-sense RNA virus belonging to the Dicistroviridae family. This natural pathogen of the model organism Drosophila melanogaster is commonly used to investigate antiviral host defense in flies, which involves both RNA interference and inducible responses. Although lethality is used routinely as a readout for the efficiency of the antiviral immune response in these studies, virus-induced pathologies in flies still are poorly understood. Here, we characterize the pathogenesis associated with systemic DCV infection. Comparison of the transcriptome of flies infected with DCV or two other positive-sense RNA viruses, Flock House virus and Sindbis virus, reveals that DCV infection, unlike those of the other two viruses, represses the expression of a large number of genes. Several of these genes are expressed specifically in the midgut and also are repressed by starvation. We show that systemic DCV infection triggers a nutritional stress in Drosophila which results from intestinal obstruction with the accumulation of peritrophic matrix at the entry of the midgut and the accumulation of the food ingested in the crop, a blind muscular food storage organ. The related virus cricket paralysis virus (CrPV), which efficiently grows in Drosophila, does not trigger this pathology. We show that DCV, but not CrPV, infects the smooth muscles surrounding the crop, causing extensive cytopathology and strongly reducing the rate of contractions. We conclude that the pathogenesis associated with systemic DCV infection results from the tropism of the virus for an important organ within the foregut of dipteran insects, the crop.
Mots clés
Animals, Dicistroviridae, growth & development, Drosophila melanogaster, virology, Female, Gastrointestinal Tract, pathology, Gene Expression Profiling, Intestinal Obstruction, virology, Muscle, Smooth, virology, Nodaviridae, growth & development, Sindbis Virus, growth & development, Viral Tropism
Référence
J. Virol.. 2014 Dec;88(24):14057-69