Tuning cell adhesive properties via layer-by-layer assembly of chitosan and alginate.
Fiche publication
Date publication
mars 2017
Journal
Acta biomaterialia
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Silva JM, García JR, Reis RL, García AJ, Mano JF
Lien Pubmed
Résumé
Understanding the mechanisms controlling cell-multilayer film interactions is crucial to the successful engineering of these coatings for biotechnological and biomedical applications. Herein, we present a strategy to tune the cell adhesive properties of multilayers based on marine polysaccharides with and without cross-linking and/or coating with extracellular matrix proteins. Chemical cross-linking of multilayers improved mechanical properties of the coatings but also elicited changes in surface chemistry that alter the adhesion of human umbilical vein endothelial cells. We evaluated a strategy to decouple the mechanical and chemical properties of these films, enabling the transition from cell-adhesive to cell-resistant multilayers. Addition of chitosan/alginate multilayers on top of cross-linked films decreased endothelial cell adhesion, spreading, and proliferation to similar levels as uncross-linked films. Our findings highlight the key role of surface chemistry in cell-multilayer film interactions, and these engineered nanocoatings represent a tunable model of cell adhesive and non-adhesive multilayered films.
Mots clés
Cell adhesion, Cross-linking, Fibronectin, Surface chemistry, Tissue engineering
Référence
Acta Biomater. 2017 03 15;51:279-293