Multilayered membranes with tuned well arrays to be used as regenerative patches.
Fiche publication
Date publication
juillet 2017
Journal
Acta biomaterialia
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CLEYMAND Franck, Pr MANO João F.
Tous les auteurs :
Martins NI, Sousa MP, Custódio CA, Pinto VC, Sousa PJ, Minas G, Cleymand F, Mano JF
Lien Pubmed
Résumé
Membranes have been explored as patches in tissue repair and regeneration, most of them presenting a flat geometry or a patterned texture at the nano/micrometer scale. Herein, a new concept of a flexible membrane featuring well arrays forming pore-like environments to accommodate cell culture is proposed. The processing of such membranes using polysaccharides is based on the production of multilayers using the layer-by-layer methodology over a patterned PDMS substrate. The detached multilayered membrane exhibits a layer of open pores at one side and a total thickness of 38±2.2µm. The photolithography technology used to produce the molds allows obtaining wells on the final membranes with a tuned shape and micro-scale precision. The influence of post-processing procedures over chitosan/alginate films with 100 double layers, including crosslinking with genipin or fibronectin immobilization, on the adhesion and proliferation of human osteoblast-like cells is also investigated. The results suggest that the presence of patterned wells affects positively cell adhesion, morphology and proliferation. In particular, it is seen that cells colonized preferentially the well regions. The geometrical features with micro to sub-millimeter patterned wells, together with the nano-scale organization of the polymeric components along the thickness of the film will allow to engineer highly versatile multilayered membranes exhibiting a pore-like microstructure in just one of the sides, that could be adaptable in the regeneration of multiple tissues.
Mots clés
Layer-by-layer, Patterning, Polyelectrolyte multilayers, Quasi-3D, Tissue engineering
Référence
Acta Biomater. 2017 07 15;57:313-323