Aneuploidy screening of embryonic stem cell clones by metaphase karyotyping and droplet digital polymerase chain reaction.
Fiche publication
Date publication
août 2016
Journal
BMC cell biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HERAULT Yann
Tous les auteurs :
Codner GF, Lindner L, Caulder A, Wattenhofer-Donzé M, Radage A, Mertz A, Eisenmann B, Mianné J, Evans EP, Beechey CV, Fray MD, Birling MC, Hérault Y, Pavlovic G, Teboul L
Lien Pubmed
Résumé
Karyotypic integrity is essential for the successful germline transmission of alleles mutated in embryonic stem (ES) cells. Classical methods for the identification of aneuploidy involve cytological analyses that are both time consuming and require rare expertise to identify mouse chromosomes.
Référence
BMC Cell Biol.. 2016 Aug;17(1):30