Human-derived extracellular matrix from Wharton's jelly: An untapped substrate to build up a standardized and homogeneous coating for vascular engineering.
Fiche publication
Date publication
janvier 2017
Journal
Acta biomaterialia
Auteurs
Membres identifiés du Cancéropôle Est :
Dr FRANCIUS Grégory, Pr DECOT Véronique, Pr MENU Patrick
Tous les auteurs :
Dan P, Velot É, Francius G, Menu P, Decot V
Lien Pubmed
Résumé
One of the outstanding goals in tissue engineering is to develop a natural coating surface which is easy to manipulate, effective for cell adhesion and fully biocompatible. The ideal surface would be derived from human tissue, perfectly controllable, and pathogen-free, thereby satisfying all of the standards of the health authorities. This paper reports an innovative approach to coating surfaces using a natural extracellular matrix (ECM) extracted from the Wharton's jelly (WJ) of the umbilical cord (referred to as WJ-ECM). We have shown by atomic force microscopy (AFM), that the deposition of WJ-ECM on surfaces is homogenous with a controllable thickness, and that this easily-prepared coating is appropriate for both the adhesion and proliferation of human mesenchymal stem cells and mature endothelial cells. Furthermore, under physiological shear stress conditions, a larger number of cells remained adhered to WJ-ECM than to a conventional coating such as collagen - a result supported by the higher expression of both integrins α2 and β1 in cells cultured on WJ-ECM. Our data clearly show that Wharton's jelly is a highly promising coating for the design of human biocompatible surfaces in tissue engineering as well as in regenerative medicine.
Mots clés
Blood Vessels, drug effects, Cell Adhesion, drug effects, Cell Differentiation, drug effects, Cell Lineage, drug effects, Cell Proliferation, drug effects, Coated Materials, Biocompatible, pharmacology, Elastic Modulus, Extracellular Matrix, drug effects, Gene Expression Regulation, drug effects, Human Umbilical Vein Endothelial Cells, drug effects, Humans, Mesenchymal Stromal Cells, cytology, Stress, Mechanical, Tissue Engineering, methods, Wharton Jelly, metabolism
Référence
Acta Biomater. 2017 01 15;48:227-237